Use of an ion trap storage/reflectron time-of-flight mass spectrometer as a rapid and sensitive detector for capillary electrophoresis in protein digest analysis.

摘要: An ion trap storage (IT)/reflectron time-of-flight mass spectrometer (reTOFMS) has been coupled to capillary electrophoresis (CE) via a sheathless microelectrospray ionization method. This hybrid proved be rapid and sensitive detector for CE, where spectra could acquired at speed sufficient maintain the high-resolution capabilities of CE separations. The nonscanning property analyzer can provide full range spectral acquisition up 25 spectra/s with data system developed in our laboratory. For work reported herein, 4 was found optimal maintaining quality separation while achieving high sensitivity. Tryptic digests bovine cytochrome c β-lactoglobulin A were analyzed using CE/IT/reTOFMS combination, resulting total electropherograms similar those obtained UV absorption detection. Taking advantage capability t...