Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design

摘要: Monkeypox virus (MPXV) infection of the prairie dog is valuable to studying systemic orthopoxvirus disease. To further characterize differences in MPXV clade pathogenesis, groups dogs were intranasally infected (8 × 10(3) p.f.u.) with Congo Basin (CB) or West African (WA) MPXV, and 28 tissues harvested on days 2, 4, 6, 9, 12, 17, 24 postinfection. Samples evaluated for presence gross microscopic lesions. Virus was recovered from nasal mucosa, oropharyngeal lymph nodes, spleen earlier CB challenged animals (day 4) than WA 6). For both groups, primary viremia (indicated by viral DNA) seen 6-9 through day 17. spread more rapidly, accumulated greater levels, caused morbidity compared MPXV. Histopathology immunohistochemistry (IHC) findings, however, similar. Two that succumbed disease demonstrated abundant antigen all organs tested, except brain. Dual-IHC staining select liver sections showed apoptotic cells (identified TUNEL) tended colocalize poxvirus antigen. Interestingly splenocytes labelled positive apoptosis often hepatocytes groups. These findings allow characterization between including identifying sites are important during early replication cellular response infection.