The effects of chloride substitution on intracellular pH in crab muscle.

摘要: 1. Intracellular pH (pHi) was measured in crab muscle fibres using pH-sensitive micro-electrodes. The mean stable pHi 7.19 +/- 00.2 (S.E. of mean) and the corresponding membrane potential -64.6 0.4 mV at an external 7.5. 2. effects on replacing 20% (100 mM) NaCl by Na salts various anions were examined. weak acid (pK'a greater than 4.5) caused large internal acidifications. strong acids less 2.6) little or no change pHi. with intermediate pK'a had varied In particular salicylate = 2.97) found to cause a fall 3. Increasing reduced It is argued that these are result entry subsequent dissociation undissociated molecules. 4. results propionate quantified comparing them 5% CO2 be smaller expected. suggested this substantial permeability anion.