Detection of amplified nucleic acid using secondary capture oligonucleotides and test kit

摘要: A target nucleic acid can be amplified and detected using a water-insoluble capture probe having short oligonucleotide (15-20 nucleotides) covalently attached to polymeric particle, one or more secondary oligonucleotides. The oligonucleotides are complementary the acid, although sequences of complementarity different. Moreover, each has length which is equal greater than oligonucleotide. hybridized either before during hybridization probe. result improved efficiency acid.