Development and validation of a neutralizer system for in vitro evaluation of some antiseptics.

摘要: A neutralizer system was developed and validated for use in the vitro bactericidal evaluation of three commonly used antiseptics, namely, Hibiclens (4% [wt/vol] chlorhexidine gluconate), Betadine (7.5% povidone-iodine), pHisoHex (3% hexachlorophene). The finally selected after a screening 12 potential candidates consisted 3% Asolectin, 10% Tween 80, 0.3% sodium thiosulfate recovery agar. This tested its neutralizing capacity as well lack inherent action against Staphylococcus aureus number gram-negative bacteria clinical significance, With no more than 10-fold dilution antiseptic, 100% effective all bacteriostatic carry-over antiseptics also completely without any test organisms used. Sodium sulfite (considered to be inactivator iodophores such Betadine), even concentrations low 0.1%, found ineffective or inherently bactericidal, whereas thiosulfate, combination with Asolectin adequate (effective non-bactericidal) considered essential neutralization Hibiclens, Betadine, pHisoHex.