Effects of cyclic nucleotides on the function of prestin.

摘要: Outer hair cells (OHCs) in the mammalian organ of Corti display electromotility, which is thought to provide local active mechanical amplification cochlear response. Prestin key molecule responsible for OHC electromotility. Several compounds, including cGMP, have been shown influence There are two potential cAMP/cGMP-dependent protein kinase phosphorylation sites on prestin. Whether these involved cGMP-dependent reactions as yet unknown. In this study, prestin cDNA was transiently transfected into TSA 201 cells. Cells that expressed were selected measure non-linear capacitance (NLC), a signature outer cell motility. We applied cGMP and cAMP analogues G (PKG) antagonist Furthermore, nine mutations at putative produced. The neutral amino acid alanine replaced serine/threonine change conserved motif order mimic dephosphorylated state prestin, whereas replacement with negatively charged aspartic mimicked phosphorylated state. properties such modified prestin-expressing examined, through measurement NLC confocal microscopy. Our data demonstrate significantly more influential than modifying non-linear, voltage-dependent charge displacement prestin-transfected electrical single double further indicate possible interaction between PKG target sites. One may membrane targeting process Finally, new topology map proposed.