High Frequency of Deletion mutations in p53 Gene from Squamous Cell Lung Cancer Patients in Taiwan

摘要: Lung cancer is the leading and second-leading cause of deaths among women men in Taiwan, respectively. However, molecular mechanisms involved lung tumorigenesis Taiwan remain poorly defined. A study that analyzed mutation spectrum p53 tumor suppressor gene 35 female patients Hong Kong showed a high proportion mutations observed were deletions, suggesting possible involvement distinct mutagenic factor(s) Chinese (Y. Takagi et al., Cancer Res., 55: 5354-5357, 1995). Therefore, to gain insight into role etiological factors we investigated spectra exons 4-11 60 Taiwan. These data also correlated with clinical pathological characteristics patients. tumors surgically resected, genomic DNA was isolated, their examined using PCR/single-strand conformational polymorphism analysis direct sequencing. The frequency 18% (11 60). patterns observed. Seven 11 detected (64%) deletions 1-12 bp at G:C or immediate vicinity repetitive sequences and/or tandem repeat sequences. In addition, two (2 11, 18%) exhibited nonsense mutations. contrast frequent occurrence missense reported literature, majority (82%) nonmissense mutations, ie., Immunohistochemical staining indicated including non-in-frame all resulted no expression protein. Notably, occurred more frequently suffering from squamous cell carcinoma (SQ). Nine 31 SQ (29%) are formation stage III IV cancer. not patients' smoking habits. Our suggest environmental genetic induced specific short may be