Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis.
作者： S. O. Dean , S. L. Rogers , N. Stuurman , R. D. Vale , J. A. Spudich
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摘要: The correct localization of myosin II to the equatorial cortex is crucial for proper cell division. Here, we examine a collection genes that cause defects in cytokinesis and reveal with live imaging two distinct phases localization. Three rho1 signaling pathway, pebble (a Rho guanidine nucleotide exchange factor), rho1, rho kinase, are required initial recruitment cortex. This mechanism does not require F-actin or components centralspindlin complex, mitotic kinesin pavarotti/MKLP1 racGAP50c/CYK-4. However, F-actin, formin (diaphanous), profilin (chickadee) stably maintain at furrow. In absence these latter genes, delocalizes from undergoes highly dynamic appearances disappearances around entire cortex, sometimes associated abnormal contractions blebbing. Our findings support model which kinase-dependent event, possibly regulatory light chain phosphorylation, furrow, whereas assembly parallel, unbranched actin filaments, generated by formin-mediated nucleation, maintaining exclusively
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