A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation

作者: José Daniel Carballeira , Blanca González-Pérez , Gabriel Moncalián , Fernando de la Cruz

DOI: 10.1093/NAR/GKU741

关键词:

摘要: … We propose that the interaction of TrwC with its cognate nic-site occurs by a highly specific lock (TrwC) and key (nic) interaction. The nic sequence contains two distinct motifs needed to …

参考文章(43)
P. Modrich, G.E. Geier, Recognition sequence of the dam methylase of Escherichia coli K12 and mode of cleavage of Dpn I endonuclease. Journal of Biological Chemistry. ,vol. 254, pp. 1408- 1413 ,(1979) , 10.1016/S0021-9258(17)34217-5
D E Bradley, D E Taylor, D R Cohen, Specification of surface mating systems among conjugative drug resistance plasmids in Escherichia coli K-12. Journal of Bacteriology. ,vol. 143, pp. 1466- 1470 ,(1980) , 10.1128/JB.143.3.1466-1470.1980
Rebekah Potts Nash, Sohrab Habibi, Yuan Cheng, Scott A. Lujan, Matthew R. Redinbo, The mechanism and control of DNA transfer by the conjugative relaxase of resistance plasmid pCU1 Nucleic Acids Research. ,vol. 38, pp. 5929- 5943 ,(2010) , 10.1093/NAR/GKQ303
Michael Chandler, Fernando de la Cruz, Fred Dyda, Alison B. Hickman, Gabriel Moncalian, Bao Ton-Hoang, Breaking and joining single-stranded DNA: the HUH endonuclease superfamily Nature Reviews Microbiology. ,vol. 11, pp. 525- 538 ,(2013) , 10.1038/NRMICRO3067
María Pilar Garcillán-Barcia, María Victoria Francia, Fernando de La Cruz, The diversity of conjugative relaxases and its application in plasmid classification Fems Microbiology Reviews. ,vol. 33, pp. 657- 687 ,(2009) , 10.1111/J.1574-6976.2009.00168.X
J. S. Edwards, L. Betts, M. L. Frazier, R. M. Pollet, S. M. Kwong, W. G. Walton, W. K. Ballentine, J. J. Huang, S. Habibi, M. Del Campo, J. L. Meier, P. B. Dervan, N. Firth, M. R. Redinbo, Molecular basis of antibiotic multiresistance transfer in Staphylococcus aureus Proceedings of the National Academy of Sciences of the United States of America. ,vol. 110, pp. 2804- 2809 ,(2013) , 10.1073/PNAS.1219701110
Remo Rohs, Sean M. West, Alona Sosinsky, Peng Liu, Richard S. Mann, Barry Honig, The role of DNA shape in protein–DNA recognition Nature. ,vol. 461, pp. 1248- 1253 ,(2009) , 10.1038/NATURE08473
María Lucas, Blanca González-Pérez, Matilde Cabezas, Gabriel Moncalian, Germán Rivas, Fernando de la Cruz, Relaxase DNA binding and cleavage are two distinguishable steps in conjugative DNA processing that involve different sequence elements of the nic site. Journal of Biological Chemistry. ,vol. 285, pp. 8918- 8926 ,(2010) , 10.1074/JBC.M109.057539
M. V. Francia, D. B. Clewell, F. de la Cruz, G. Moncalian, Catalytic domain of plasmid pAD1 relaxase TraX defines a group of relaxases related to restriction endonucleases Proceedings of the National Academy of Sciences of the United States of America. ,vol. 110, pp. 13606- 13611 ,(2013) , 10.1073/PNAS.1310037110