Introduction of anaerobic dechlorinating bacteria into soil slurry microcosms and nested-PCR monitoring.

摘要: Desulfomonile tiedjei and Desulfitobacterium dehalogenans were chosen as model bacteria to demonstrate the introduction of an anaerobic microbia reductive dechlorination activity into nonsterile soil slurry microcosms by inoculation. De novo 3-chlorobenzoate was established with bacterium D. in normally devoid this capacity. The addition supplemented 20 mM pyruvate cosubstrate resulted total biotransformation 1.5 within 7 days. a shortening period required for be established. In inoculated dehalogenans, degradation 6 3-chloro-4-hydroxy phenoxyacetic acid (3-Cl-4-OHPA) observed after 4 days contrast result noninoculated microcosms, where 3-Cl-4-OHPA indigenous microorganisms 11 Both externally introduced bacterial strains detected nested-PCR methodology.