Identification of a Novel Reference Gene for Apple Transcriptional Profiling under Postharvest Conditions

摘要: Reverse Transcription quantitative PCR (RT-qPCR) is one of the most important techniques for gene expression profiling due to its high sensibility and reproducibility. However, reliability results highly dependent on data normalization, performed by comparisons between profiles genes interest against those constitutively expressed, reference genes. Although technique widely used in fruit postharvest experiments, transcription stability has not been thoroughly investigated under these experimental conditions. Thus, we have determined transcriptional profile, conditions, three commonly as reference—ACTIN (MdACT), PROTEIN DISULPHIDE ISOMERASE (MdPDI) UBIQUITIN-CONJUGATING ENZYME E2 (MdUBC)—along with two novel candidates—HISTONE 1 (MdH1) NUCLEOSSOME ASSEMBLY (MdNAP1). The profile was throughout five them encompassing period other two, consisting developmental spatial phases. comparatively using four distinct software packages: BestKeeper, NormFinder, geNorm DataAssist. Gene ranking were similar packages, exception BestKeeper. classic MdUBC ranked among stably transcribed all Transcript accumulation candidate MdH1 stable tested especially experiments period. our present a apple reinforce importance checking conditions interest.