PRODUCTION AND CHARACTERIZATION OF MULTIPLE-LAYERED POPULATIONS OF ANIMAL CELLS

摘要: Dense populations containing 129 x 106 Jensen sarcoma, 134 DON Chinese hamster, 28.9 WI-38 human diploid, 61.8 HEp-2 carcinoma, and 67.4 WISH amnion cells were produced from dilute inocula, 0.85 to 5.33 106, in 7 8 days a perfusion system using replicate T-60 flasks. Perfusion rates as high 560 ml medium/day/T-60 required maintain pH (to ca ±0.1 unit) adequate nutrient supplies. The cell densities encountered are described by the term "monolayer equivalents" (M.E.), defined number of per culture divided monolayer. M.E.'s for cultures unusually dense 40 250 (9-day perfusion) 5 17, respectively, numbers illustrations stained cross-sections membranes these excellent agreement. Threshold exist below which proliferation is chief cellular activity above one or more functions may predominate even though persists. Cellular nutrition metabolism change with changes M.E., illustrated different patterns glutamic acid, proline, glycine utilization production vs. populations. results indicated that role contact inhibition phenomena arresting was diminished environments.