Analysis of neurons created from wild-type and Alzheimer's mutation knock-in embryonic stem cells by a highly efficient differentiation protocol
作者： Yoichiro Abe , Keisuke Kouyama , Taisuke Tomita , Yusuke Tomita , Norimitsu Ban
DOI: 10.1523/JNEUROSCI.23-24-08513.2003
关键词:
摘要: It is impossible to obtain and amplify live neurons from Alzheimer's disease (AD) patients. To establish the harboring AD abnormality, we constructed mouse embryonic stem (ES) cells, in which AD-causative V642I mutation was introduced endogenous amyloid precursor protein ( APP ) gene, combination with a protocol efficiently differentiate ES cells into postmitotic without using cell sorter. By this protocol, differentiated >90% of central type adult neurons. Neurons derived V642I–APP knock-in were indistinguishable wild-type ES-derived neurons, as determined by expression various markers for neuronal differentiation. Notably, cell-derived exhibited significantly increased secretion Aβ42 AD-related hyperphosphorylation tau, indicating that direct output secretion. In study, analyze created genotypes propose new strategy generating any dominantly inherited neurodegenerative diseases. The can be applied create human or other cells.
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