Antimicrobial peptides from Mirabilis jalapa and Amaranthus caudatus: expression, processing, localization and biological activity in transgenic tobacco
作者： Miguel F. C. De Bolle , Rupert W. Osborn , Inge J. Goderis , Liesbet Noe , David Acland
DOI: 10.1007/BF00040718
关键词:
摘要: The cDNAs encoding the seed antimicrobial peptides (AMPs) from Mirabilis jalapa (Mj-AMP2) and Amaranthus caudatus (Ac-AMP2) have previously been characterized it was found that Mj-AMP2 Ac-AMP2 are processed a precursor preprotein preproprotein, respectively [De Bolle et al., Plant Mol Biol 28:713-721 (1995) 22:1187-1190 (1993), respectively]. In order to study processing, sorting biological activity of these in transgenic tobacco, four different gene constructs were made: wild-type construct, mutant construct which extended by sequence barley lectin carboxyl-terminal propeptide, known vacuolar targeting signal [Bednarek Raikhel, Cell 3: 1195-1206 (1991)]; an construct; finally, truncated delete genuine propeptide. Processing localization analysis indicated isoform with cleaved-off arginine localized intercellular fluid fraction plants expressing either or constructs. recovered extracellularly transformed whereas accumulated intracellularly protein vitro antifungal AMPs purified tobacco any proteins similar authentic proteins. However, none showed enhanced resistance against infection Botrytis cinerea Alternaria longipes.
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sci-hub.se 参考文章(58)
R. Deblaere, A. Reynaerts, H. Höfte, J.-P. Hernalsteens, J. Leemans, M. Van Montagu, [16] Vectors for cloning in plant cells Methods in Enzymology. ,vol. 153, pp. 277- 292 ,(1987) , 10.1016/0076-6879(87)53059-2
Miguel F. C. De Bolle, Franky R. G. Terras, Bruno P. A. Cammue, Sarah B. Rees, Willem F. Broekaert, Mirabilis Jalapa Antibacterial Peptides and Raphanus Sativus Antifungal Proteins: A Comparative Study of their Structure and Biological Activities Springer, Dordrecht. pp. 433- 436 ,(1993) , 10.1007/978-94-011-1737-1_128
G. An, B.D. Watson, S. Stachel, M.P. Gordon, E.W. Nester, New cloning vehicles for transformation of higher plants. The EMBO Journal. ,vol. 4, pp. 277- 284 ,(1985) , 10.1002/J.1460-2075.1985.TB03626.X
R. Fernandez De Caleya, Blanca Gonzalez-Pascual, F. García-Olmedo, Pilar Carbonero, Susceptibility of Phytopathogenic Bacteria to Wheat Purothionins In Vitro Applied Microbiology. ,vol. 23, pp. 998- 1000 ,(1972) , 10.1128/AM.23.5.998-1000.1972
Willem F. Broekaert, Frankt R.G. Terras, Bruno P.A. Cammue, Jos Vanderleyden, An automated quantitative assay for fungal growth inhibition Fems Microbiology Letters. ,vol. 69, pp. 55- 59 ,(1990) , 10.1111/J.1574-6968.1990.TB04174.X
Marja C.P. Timmermans, Pal Maliga, Jeffrey Vieira, Joachim Messing, The pFF plasmids: cassettes utilising CaMV sequences for expression of foreign genes in plants. Journal of Biotechnology. ,vol. 14, pp. 333- 344 ,(1990) , 10.1016/0168-1656(90)90117-T
W. F. Broekaert, FRG. Terras, BPA. Cammue, R. W. Osborn, Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiology. ,vol. 108, pp. 1353- 1358 ,(1995) , 10.1104/PP.108.4.1353
S Y Bednarek, T A Wilkins, J E Dombrowski, N V Raikhel, A carboxyl-terminal propeptide is necessary for proper sorting of barley lectin to vacuoles of tobacco. The Plant Cell. ,vol. 2, pp. 1145- 1155 ,(1990) , 10.1105/TPC.2.12.1145
A. Hoekema, P. R. Hirsch, P. J. J. Hooykaas, R. A. Schilperoort, A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid Nature. ,vol. 303, pp. 179- 180 ,(1983) , 10.1038/303179A0
D. Liu, K. G. Raghothama, P. M. Hasegawa, R. A. Bressan, Osmotin overexpression in potato delays development of disease symptoms. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 1888- 1892 ,(1994) , 10.1073/PNAS.91.5.1888