The polymerase chain reaction method as a tool for identifying members of the Anopheles gambiae complex (Diptera:Culicidae) in northeastern Tanzania.

摘要: Polymerase chain reaction (PCR) primers developed at the Centers for Disease Control in Atlanta identification of members Anopheles (Cellia) gambiae Giles complex were tested on material collected Bagamoyo and Muheza districts northeastern Tanzania. Part sample from was chromosomally identified correlated with PCR identifications. This contained 170 arabiensis, 328 An. gambiae, 58 merus, which 121, 237, 54 specimens, respectively, both chromosomes. Three specimens as merus gave only fragment characteristic quadriannulatus, but retesting correct result. The consisted 771 852 43 4 producing quadriannulatus. Because quadriannulatus has never been recorded mainland Tanzania due to high number that produced no result (193), it is probable DNA degradation led misidentification overall probability by 99.685% first testing, compares favorably other genetic methods currently use.