Anatomically selective peripheral nerve ablation using intraneural ricin injection

摘要: Anatomically selective destruction of sensory and motor neurons based upon which nerve contains the corresponding axons can be accomplished by intraneural pressure microinjection toxic lectin, ricin. Ricin is taken up at injection site axonally transported to perikarya resulting in neurons. In present report, we describe a reliable procedure for making such lesions using ricin into trunks. Consistent, complete restricted appropriate are documented after vagus, hypoglossal, phrenic sciatic nerves superior cervical ganglion. Complete vagal ablations could achieved with 100 ng or less ricin; whereas, 1-3 micrograms was required obtain similar results hypoglossal nerves. Although most dead within 24 h injection, survival times 10-14 days may necessary disappearance poisoned This technique valuable highly anatomical, neurochemical neurophysiological experiments.