The wheat mitochondrial rps13 gene: RNA editing and co-transcription with the atp6 gene.

摘要: Northern analyses and reverse transcription-polymerase chain-reaction (RT-PCR) experiments, followed by PCR amplification product sequencing, were performed on total mitochondrial (mt) RNAs from wheat seedlings tissue cultures. It was shown that the rps13 gene, which encodes ribosomal protein S13, atp6 subunit 6 of ATP synthase complex, co-transcribed. However, transcripts virtually undetectable in under conditions where appeared abundant. In addition, markedly higher steady state transcript levels observed culture. Expression gene confirmed showing its edited. Slight differences between editing patterns tissue-culture whole-plant found. Taken together, these results suggest vitro culture could disturb post-transcriptional regulation expression.