Zinc Ion-Dependent Peptide Nucleic Acid-Based Artificial Enzyme that Cleaves RNA-Bulge Size and Sequence Dependence.
作者： Merita Murtola , Alice Ghidini , Pasi Virta , Roger Strömberg
DOI: 10.3390/MOLECULES22111856
关键词:
摘要: In this report, we investigate the efficiency and selectivity of a Zn2+-dependent peptide nucleic acid-based artificial ribonuclease (PNAzyme) that cleaves RNA target sequences. The RNAs are varied to form different sizes (3 4 nucleotides, nt) sequences in bulge formed upon binding PNAzyme. PNAzyme-promoted cleavage was observed variation substrate showed clear dependence on sequence size bulge. For targets 4-nt bulges, identified systems with an improved efficacy (an estimated half-life ca 7–8 h as compared 11–12 for studied earlier) well site (up over 70% at single 50–60% previous sequences). forming 3-nt enhancement even more pronounced. Compared starting point AAA bulges (half-lives 21–24 h), could identify were cleaved half-lives three times lower (ca i.e., rates similar those found fastest system. addition, further reach 80% specific site.
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