Investigation of Candida albicans transmission in a surgical intensive care unit cluster by using genomic DNA typing methods.

摘要: An apparent outbreak of serious Candida albicans infections (n = 6) occurred in a surgical intensive care unit over 4-week period. Four patients developed C. bloodstream infections. additional patient catheter-related infection; the sixth an infection cerebrospinal fluid. was isolated from hands five health workers (17%) and throat one worker (3%) during investigation. Karyotyping restriction endonuclease analysis genomic DNA with BssHII 23 isolates 6 revealed 9 12 different patterns, respectively. Three six appeared to be infected same strain (two fluid infection). The were colonized that identical isolate prior patient. However, SfiI found differences among determined by other two methods. alone does not appear sufficient differentiate between control isolates. Restriction typing may more sensitive method than karyotyping investigation cluster Furthermore, use enzyme necessary for optimal discrimination DNA.