Ultrastructure in anterior and posterior stroma of perfused human and rabbit corneas. Relation to transparency.

摘要: Purpose. The authors sought to discover whether there are differences in the degree of spatial order fibrillar ultrastructure between anterior and posterior stroma. Methods. Human corneas were obtained from eye bank eyes. Although they had been classified as normal, some swelling remained after 3 hours deturgescence. Freshly excised, unswollen rabbit also used. Image analysis methods applied transmission electron micrographs anterior, middle, stroma these determine positions radii fibrils, fraction total area occupied by fibril number density. Results used calculate interference factor that appears direct summation fields for light scattering theory estimate cross-section per fibril. is a measure sizes fibrils. Results. Electron showed anterior-posterior variations size density at wavelengths visible was lower than humans rabbits. In instances humans, characteristic mildly swollen cornea. When averaged analyzed, predicted scatter approximately twice much unit depth (at any given wavelength) three times Conclusions. Calculations ordering human corneas, could have caused intrinsic vivo ; however, possible two regions vitro affected results. Invest Ophthalmol Vis Sci. 1995 ;36 :1508-1523.