Role of gibberellins during arbuscular mycorrhizal formation in tomato: new insights revealed by endogenous quantification and genetic analysis of their metabolism in mycorrhizal roots

摘要: Gibberellins (GAs) are key regulators of plant growth and development recent studies suggest also a role during arbuscular mycorrhizal (AM) formation. Here, complementary approaches have been used to obtain clearer picture that correlates AM fungal inside roots with GA metabolism. An extensive analysis genes associated metabolism as well quantification content in was made. Application GA3 its biosynthesis inhibitor prohexadione calcium (PrCa) combined GA-constitutive response mutant (procera) were determine whether colonization is altered by the level these hormones or changes GA-signaling pathway. The increased levels specific GAs from 13-hydroxylation pathway correlate closely expression coding enzymes biosynthetic trail. imbalance tomato caused exogenous applications PrCa affects arbuscules both negative positive ways, respectively. In addition, procera plants adversely affected mycorrhization process. Our findings demonstrate an favor amount negatively frequency particularly abundance results point out formation change GAs.