摘要: The increasing demand for DNA sequences can be met by replacement of each sample in a device with mixture N samples so that the normal throughput is increased factor N. Such method described. In order to separate sequence information at end processing, molecules interest are ligated set oligonucleotide "tags" beginning. tagged pooled, amplified, and chemically fragmented 96-well plates. resulting reaction products fractionated size on sequencing gels transferred nylon membranes. These membranes then probed as many times there types tags original pools, producing, cycle probing, autoradiographs similar those from standard methods. Thus, gel yields quantity data equivalent obtained conventional reactions multiplied number probes used. To date, even after 50 successive probings, signal strength image quality retained, an indication upper limit reprobings may considerably higher.
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