Acetate peel technique: a rapid way of preparing sequential surface replicas of dental hard tissues for microscopic examination.

摘要: Summery Objective: In order to study dental structures in three-dimensional views, sequential microscopic sections are needed. Routine thin-sectioning needs equipment, it is time-consuming, expensive and cause sample wastage during sectioning. The acetate peel technique used for examinations of carbonate rocks fossils was modified successfully adapted teeth. Design: A human tooth embedded resin block; the surface be peeled ground polished with carborundum powder on a glass plate using water. etched, washed, dried. dry flooded acetone piece film placed over it. As evaporated, settled down take shape micro relief produced by etching. dried 10–15 min. It pulled off mounted between two slides examination under microscope. Results: This enabled making numerous replicas (peels) from tooth, especially fully mineralised enamel without routine decalcifying, dehydrating, sawing, mounting processes. Conclusions: results obtained stained peels, showed that this an efficient way gross fine etched hard tissues surfaces restoration-cavity marginal integrity detail.