Sequence organisation and transcriptional regulation of the mouse elastase II and trypsln genes

摘要: Elastase II and trypsin mRNAs were cloned in form of their cDNAs from pancreas strain A/J mice, complete nucleotide sequences determined. The elastase mRNA is 912 nucleotides long encodes a protein 271 amino acids. species 814 246 gene, which exists as single copy the haploid mouse genome, measures 11.2 kb cap to poly(A) site interrupted by at least seven introns. Between 5 10 genes exist genome. Five different genes, two are closely linked tail-to-tail manner, studied detail. They vary size between 3.4 4.0kb, all four DNA sequence comparison II, Amy-2a alpha-amylase reveals conserved 13 motif 5'-flanking regions. differential accumulation cytoplasm acinar pancreatic cell regulated predominantly transcriptional level.