MicroRNA-30a regulates zebrafish myogenesis through targeting the transcription factor Six1

摘要: Precise spatiotemporal regulation of the SIX1 homeoprotein is required to coordinate vital tissue development, including myogenesis. Whereas downregulated in most tissues following embryogenesis, it re-expressed numerous cancers, tumors derived from muscle progenitors. Despite crucial roles development and disease, upstream expression has remained elusive. Here, we identify first direct mechanism for Six1 through microRNA30a (miR30a)-mediated repression. In zebrafish somites, show that miR30a six1a six1b (hereafter six1a/b) are expressed an inverse temporal pattern. Overexpression leads a reduction six1a/b levels, results increased apoptosis altered somite morphology, which phenocopies knockdown. Conversely, inhibition abnormal revealing role endogenous as muscle-specific miRNA (myomiR). Importantly, restoration miR30a-overexpressing embryos restores proper These data demonstrate new at key node myogenic regulatory gene network controlling expression.