Enhanced VEGF/VEGF-R and RUNX2 Expression in Human Periodontal Ligament Stem Cells Cultured on Sandblasted/Etched Titanium Disk.

摘要: Bone formation, in skeletal development or osseointegration processes, is the result of interaction between angiogenesis and osteogenesis. To establish osseointegration, cells must attach to implant a direct way without any deposition soft tissue. Structural design surface topography dental implants enhance cell attachment can affect biological response. The aim study was evaluate cytocompatibility, osteogenic angiogenic markers involved bone differentiation human periodontal ligament stem (hPDLSCs) on different titanium disks surfaces. hPDLSCs were cultured pure surfaces modified with two procedures, sandblasted (Control-CTRL) sandblasted/etched (Test-TEST) as experimental After 1 8 weeks culture VEGF, VEGF-R, RUNX2 expression evaluated under confocal laser scanning microscopy. confirm obtained data, RT-PCR WB analyses performed order best performance. TEST compared CTRL enhanced adhesion increased VEGF expression. Moreover, showed topographic morphology that promoted adhesion, proliferation, osteogenic/angiogenic commitment. conclude, more efficiently than surfaces; furthermore, results them be biocompatible, better tolerated, appropriate for allowing hPDLSC growth proliferation. This fact could also lead rapid bone-titanium integration.