A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein.

摘要: Effector proteins are translocated into host cells by plant-pathogens to undermine pattern-triggered immunity (PTI), the plant response microbe-associated molecular patterns that interferes with infection process. Individual effectors found in variable repertoires where some constituents target same pathways. The effector protein AvrPto from Pseudomonas syringae has a core domain (CD) and C-terminal (CTD) each promotes bacterial growth virulence tomato. individual contributions of whether they act redundantly is unknown. We use RNA-Seq elucidate contribution CD CTD suppression PTI tomato leaves 6 h after inoculation. Unexpectedly, alters transcript levels essentially genes but different degree. This difference, when quantified, reveals although targeting genes, two domains synergistically. relatively greater effect on whose expression suppressed during PTI, these appears be diminished saturation. profiles can used observe relative subdomains suppression. Our analysis shows multiplicatively affect gene impact PTI. higher degree up-regulation versus down-regulation plausibly an evolutionary adaptation against immune signaling.