作者: Y. Deb , D. Marti , M. Frenz , C. Kuhlemeier , D. Reinhardt
DOI: 10.1242/DEV.121244
关键词:
摘要: The spatial arrangement of leaves and flowers around the stem, known as phyllotaxis, is controlled by an auxin-dependent reiterative mechanism that leads to regular spacing organs thereby remarkably precise phyllotactic patterns. based on active cellular transport phytohormone auxin influx efflux carriers, such AUX1 PIN1. Their important role in phyllotaxis evident from mutant phenotypes, but their exact roles space time are difficult address due strong pleiotropic phenotypes most mutants phyllotaxis. Models invoke accumulation at leaf initials removal through developing vascular strand, midvein. We have developed a microsurgical tool ablate midvein high temporal resolution order test its function formation Using amplified femtosecond laser pulses, we ablated internal tissues young primordia tomato (Solanum lycopersicum) without damaging overlying L1 L2 layers. Our results show ablation future transient increase width. Phyllotaxis was transiently affected after ablations, readjusted two plastochrons. These indicate involved basipetal primordia, which contributes stability.