Ginkgolic acid inhibits HIV protease activity and HIV infection in vitro.

摘要: Background Several HIV protease mutations, which are resistant to clinical inhibitors (PIs), have been identified. There is a great need for second-generation PIs with different chemical structures and/or an alternative mode of inhibition. Ginkgolic acid natural herbal substance and major component the lipid fraction in nutshells Ginkgo biloba tree. The objective this study was determine whether ginkgolic could inhibit activity cell free system infection human cells. Material/methods Purified recombinant HIV-1 HXB2 KIIA were used assay. Human peripheral blood mononuclear cells (PBMCs) (HIV-1SF162 virus), determined by p24gag ELISA. Cytotoxicity also determined. Results (31.2 µg/ml) inhibited 60%, compared negative control, effect concentration-dependent. In addition, treatment (50 100 effectively at day 7 concentration-dependent manner. concentration up 150 µg/ml demonstrated very limited cytotoxicity. Conclusions inhibits PBMCs without significant may through mechanisms than current FDA-approved PI drugs. These properties make it promising therapy infection, especially as problem viral resistance continues grow.