Oligonucleotide primers for DNA amplification of the early regions 1, 6, and 7 from human papillomavirus types 6, 11, 16, 18, 31, and 33.

作者: M. Evander , E. Bod�n , L. Bjersing , E. Rylander , G. Wadell

DOI: 10.1007/BF01319244

关键词:

摘要: Human papillomavirus (HPV) type-specific sequences required for polymerase chain reaction (PCR) mediated amplification of HPV DNA are presented. One primer pair within the E1 open reading frame (ORF) was shared by 6, 11, 16, and 31, whereas other ORF specific 16. Eight pairs from E6 E7 ORFs specifically detected 18, 33 sequences. This system has been used detection in biopsies, cytological smears sections formalin-fixed tissues.

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