Paired Heavy and Light Chain Signatures Contribute to Potent SARS-CoV-2 Neutralization in Public Antibody Responses
作者： Bailey B. Banach , Gabriele Cerutti , Ahmed S. Fahad , Chen-Hsiang Shen , Matheus Olivera de Souza
DOI: 10.2139/SSRN.3754549
关键词:
摘要: Understanding protective mechanisms of antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We discovered a new antibody, 910-30, that targets the SARS-CoV-2 ACE2 receptor binding site as member public response encoded by IGHV3-53/IGHV3-66 genes. performed sequence structural analyses to explore how features correlate with neutralization. Cryo-EM structures 910-30 bound spike trimer revealed its interactions ability disassemble spike. Despite heavy chain similarity, biophysical IGHV3-53/3-66 antibodies highlighted importance native heavy:light pairings for competition defined paired signatures determined precursor prevalence be ~1 in 44,000 human B cells, consistent identification several convalescent COVID-19 patients. These data reveal key functional neutralization class accelerate antibody-based efforts
sci-hub.st 参考文章(85)
R. C. Ezeokonkwo, W. E. Agu, S.J. Black, Non-radioactive method for labelling Trypanosoma brucei brucei (S427, Clone 22) surface proteins using EZ-Link Sulfo-NHS-LC-Biotin (Sulfosuccinimidyl-6- (Biotinamido) Hexanoate) Tropical veterinarian. ,vol. 21, pp. 14- 22 ,(2003) , 10.4314/TV.V21I1.4523
Benjamin A Barad, Nathaniel Echols, Ray Yu-Ruei Wang, Yifan Cheng, Frank DiMaio, Paul D Adams, James S Fraser, EMRinger: side chain–directed model and map validation for 3D cryo-electron microscopy Nature Methods. ,vol. 12, pp. 943- 946 ,(2015) , 10.1038/NMETH.3541
Christopher O. Barnes, Anthony P. West, Kathryn E. Huey-Tubman, Magnus A.G. Hoffmann, Naima G. Sharaf, Pauline R. Hoffman, Nicholas Koranda, Harry B. Gristick, Christian Gaebler, Frauke Muecksch, Julio C. Cetrulo Lorenzi, Shlomo Finkin, Thomas Hagglof, Arlene Hurley, Katrina G. Millard, Yiska Weisblum, Fabian Schmidt, Theodora Hatziioannou, Paul D. Bieniasz, Marina Caskey, Davide F. Robbiani, Michel C. Nussenzweig, Pamela J. Bjorkman, Structures of human antibodies bound to SARS-CoV-2 spike reveal common epitopes and recurrent features of antibodies. bioRxiv. ,(2020) , 10.1101/2020.05.28.121533
Lorenzo Benatuil, Jennifer M. Perez, Jonathan Belk, Chung-Ming Hsieh, An improved yeast transformation method for the generation of very large human antibody libraries Protein Engineering Design & Selection. ,vol. 23, pp. 155- 159 ,(2010) , 10.1093/PROTEIN/GZQ002
Eric T. Boder, K. Dane Wittrup, Yeast surface display for screening combinatorial polypeptide libraries Nature Biotechnology. ,vol. 15, pp. 553- 557 ,(1997) , 10.1038/NBT0697-553
Andreas Bräuninger, Tina Goossens, Klaus Rajewsky, Ralf Küppers, Regulation of immunoglobulin light chain gene rearrangements during early B cell development in the human. European Journal of Immunology. ,vol. 31, pp. 3631- 3637 ,(2001) , 10.1002/1521-4141(200112)31:12<3631::AID-IMMU3631>3.0.CO;2-L
Philip J.M. Brouwer, Tom G. Caniels, Karlijn van der Straten, Jonne L. Snitselaar, Yoann Aldon, Sandhya Bangaru, Jonathan L. Torres, Nisreen M.A. Okba, Mathieu Claireaux, Gius Kerster, Arthur E.H. Bentlage, Marlies M. van Haaren, Denise Guerra, Judith A. Burger, Edith E. Schermer, Kirsten D. Verheul, Niels van der Velde, Alex van der Kooi, Jelle van Schooten, Mariëlle J. van Breemen, Tom P. L. Bijl, Kwinten Sliepen, Aafke Aartse, Ronald Derking, Ilja Bontjer, Neeltje A. Kootstra, W. Joost Wiersinga, Gestur Vidarsson, Bart L. Haagmans, Andrew B. Ward, Godelieve J. de Bree, Rogier W. Sanders, Marit J. van Gils, Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability bioRxiv. ,(2020) , 10.1101/2020.05.12.088716
Yongfei Cai, Jun Zhang, Tianshu Xiao, Hanqin Peng, Sarah M. Sterling, Richard M. Walsh, Shaun Rawson, Sophia Rits-Volloch, Bing Chen, Distinct conformational states of SARS-CoV-2 spike protein Science. ,vol. 369, pp. 1586- 1592 ,(2020) , 10.1126/SCIENCE.ABD4251
Yunlong Cao, Bin Su, Xianghua Guo, Wenjie Sun, Yongqiang Deng, Linlin Bao, Qinyu Zhu, Xu Zhang, Yinghui Zheng, Chenyang Geng, Xiaoran Chai, Runsheng He, Xiaofeng Li, Qi Lv, Hua Zhu, Wei Deng, Yanfeng Xu, Yanjun Wang, Luxin Qiao, Yafang Tan, Liyang Song, Guopeng Wang, Xiaoxia Du, Ning Gao, Jiangning Liu, Junyu Xiao, Xiao-dong Su, Zongmin Du, Yingmei Feng, Chuan Qin, Chengfeng Qin, Ronghua Jin, X. Sunney Xie, Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells. Cell. ,vol. 182, ,(2020) , 10.1016/J.CELL.2020.05.025
Jovani Catalan-Dibene, Human antibodies can neutralize SARS-CoV-2. Nature Reviews Immunology. ,vol. 20, pp. 350- 350 ,(2020) , 10.1038/S41577-020-0313-6