Mismatch Repair mRNA and Protein Expression in Intestinal Adenocarcinoma in Sika Deer (Cervus nippon) Resembling Heritable Non-polyposis Colorectal Cancer in Man

摘要: Summary Intestinal adenocarcinomas seen in an inbred herd of farmed sika deer ( Cervus nippon ) morphologically resembled human hereditary non-polyposis colorectal cancer (HNPCC). Features common to both included multiple de novo sites tumourigenesis the proximal colon, sessile and mucosal changes, frequent finding mucinous type adenocarcinoma, lymphocyte infiltration into neoplastic tubules Crohn's-like lymphoid follicles at deep margin tumour. HNPCC is defined by a germline mutation mismatch repair (MMR) genes resulting their inactivation loss expression. To test hypothesis that similar MMR gene occurs tumours, expression four most important genes, MSH2 , MLH1 MSH6 PMS2 was examined mRNA level reverse transcriptase polymerase chain reaction n  = 12) protein immunohistochemistry  = 40) tumour control tissues. All were expressed equally normal tissues, so could not be implicated carcinogenesis this deer.