Protein Assembly on Membrane Surface alters the Dynamical Spectrum of Downstream Signaling Reactions

摘要: Protein-protein interactions have been well documented to give rise an elaborated spatial pattern of signaling molecules during signal transduction. In the case T-cell receptor (TCR) signaling, multivalent at linker for activation (LAT) promote assembly structure with downstream on membrane surface upon TCR activation. However, perturbation dynamics remains largely unknown. We reconstituted geometry LAT minimum components in supported membranes. With single-molecule analysis, we directly observe dynamic alteration reactions by structure. The consequence altered kinetics is further analyzed analytical calculations. Together, propose assembly-dependent mechanism triggering, which can be a general statement other receptors interactions.