Use of Antibodies to Purify Cholinergic Nerve Terminals
作者： Peter J. Richardson
DOI: 10.1007/978-1-4684-5006-4_23
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摘要: In the isolation of nerve terminals from mammalian CNS — crucial for elucidating synaptic biochemistry [1] a major limitation is heterogeneity preparations, particularly diversity distinct neuronal types present. Previous attempts to purify neurotransmitter-specific were frustrated by similarities in density and size most [2], while absence known transmitter-specific surface antigens prevented application immunological techniques.
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