Rates of Aziridinium Alkylation of Polynucleotides and Ribonucleic Acid

摘要: The exceptionally high rate of alkylation guanine units in double helix DNA (k g /kw1 = 150,000) (P rice , C. C., G aucher G. M., K oneru P., S hibakawa R., owa J. and Y amaguchi M. (1968) Biochim. Biophys. Acta 166, 327) has now been observed for tRNA /kw 97,000), rRNA 93,000), a 1:1 mixture polyguanylic polycytidylic acid 80,000). unreactivity adenine (1) also found polyadenylic polyuridylic acids. Poly(A), poly(C), poly(G) all show markedly greater base reactivity than their monomers, ascribed partly to enhanced from stacking the bases polymers. Poly(G), tRNA, showed sharp changes after 5 10% reaction, interpreted as due “denaturation” or degradation resulting and/or phosphate groups. Very initial reactivities (kp/kw ≃ 106) groups, poly(A), (A)n·(U)n, rRNA, are electrostatic effects, neutralized proceeds.