Transcription of polyoma virus DNA in vitro: III. Localization of calf thymus RNA polymerase II binding sites

摘要: Abstract The binding sites of calf thymus RNA polymerase II on polyoma DNA were monitored by electron microscopy. Six discrete located at positions 0.06, 0.25, 0.57, 0.66, 0.85 and 0.98 the physical map DNA. Although most these are in easily denaturable regions DNA, strongest do not overlap with major A + T-rich regions. In addition, same observed superhelical or linear These results suggest that eucaryotic can recognize specific sequences double-stranded only At least five correspond to initiation mapped previously for Escherichia coli (Lescure et al. , 1976). Stable complexes be formed both E. polymerases presence a single dinucleotide (GpU) ribotriphosphate (CTP). Under conditions, enzymes 0.06 0.57 is stimulated whereas 0.65 0.84 partially suppressed. Both procaryotic may similar viral vitro .