Structure and function of Vps15 in the endosomal G protein signaling pathway.

摘要: G protein-coupled receptors mediate cellular responses to a wide variety of stimuli, including taste, light, and neurotransmitters. In the yeast Saccharomyces cerevisiae, activation pheromone pathway triggers events leading mating. The view had long been held that protein-mediated signal occurs principally at plasma membrane. Recently, it has shown protein α subunit Gpa1 can promote signaling endosomes requires two components sole phosphatidylinositol-3-kinase in yeast, Vps15 Vps34. contains multiple WD repeats also binds preferentially GDP-bound state; these observations led us hypothesize may function as β endosome. Here we show an X-ray crystal structure domain reveals seven-bladed propeller resembling typical Gβ subunits. We further is sufficient bind well Atg14, potential Gγ exists complex wi...