Salmonella maintains the integrity of its intracellular vacuole through the action of SifA.
作者： Carmen R Beuzón , Stéphane Méresse , Kate E Unsworth , Javier Ruíz-Albert , Steve Garvis
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摘要: A method based on the Competitive Index was used to identify Salmonella typhimurium virulence gene interactions during systemic infections of mice. Analysis mixed involving single and double mutant strains showed that OmpR, type III secretion system pathogenicity island 2 (SPI-2) SifA [required for formation in epithelial cells lysosomal glycoprotein (lgp)-containing structures, termed Sifs] are all involved same function. sifA expression induced after entry into host dependent SPI-2 regulator ssrA. sifA(-) strain had a replication defect macrophages, similar ompR(-) strains. Whereas wild-type reside vacuoles progressively acquire lgps vacuolar ATPase, majority bacteria lost their membrane were released cell cytosol. We propose strain, through action effectors (including SpiC), diverts Salmonella-containing vacuole from endocytic pathway, subsequent recruitment maintenance ATPase/lgp-containing membranes enclose replicating is mediated by translocation SifA.
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sci-hub.se 参考文章(62)
Carmen R. Beuzon, Geoff Banks, Jorg Deiwick, Michael Hensel, David W. Holden, PH-DEPENDENT SECRETION OF SSEB, A PRODUCT OF THE SPI-2 TYPE III SECRETION SYSTEM OF SALMONELLA TYPHIMURIUM Molecular Microbiology. ,vol. 33, pp. 806- 816 ,(1999) , 10.1046/J.1365-2958.1999.01527.X
Zheng Zhang, Webb Miller, David J Lipman, Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research. ,vol. 25, pp. 3389- 3402 ,(1997) , 10.1093/NAR/25.17.3389
Murry A Stein, Ka Yin Leung, Michael Zwick, Francisco Garcia‐del Portillo, B Brett Finlay, None, Identification of a Salmonella virulence gene required for formation of filamentous structures containing lysosomal membrane glycoproteins within epithelial cells Molecular Microbiology. ,vol. 20, pp. 151- 164 ,(1996) , 10.1111/J.1365-2958.1996.TB02497.X
H. Ochman, F. C. Soncini, F. Solomon, E. A. Groisman, Identification of a pathogenicity island required for Salmonella survival in host cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 7800- 7804 ,(1996) , 10.1073/PNAS.93.15.7800
Stéphane Méresse, Olivia Steele‐Mortimer, B Brett Finlay, Jean‐Pierre Gorvel, The rab7 GTPase controls the maturation of Salmonella typhimurium-containing vacuoles in HeLa cells The EMBO Journal. ,vol. 18, pp. 4394- 4403 ,(1999) , 10.1093/EMBOJ/18.16.4394
Raphael H. Valdivia, Stanley Falkow, Fluorescence-Based Isolation of Bacterial Genes Expressed Within Host Cells Science. ,vol. 277, pp. 2007- 2011 ,(1997) , 10.1126/SCIENCE.277.5334.2007
Finlay Bb, Garcia-del Portillo F, Zwick Mb, Leung Ky, Intracellular replication of Salmonella within epithelial cells is associated with filamentous structures containing lysosomal membrane glycoproteins. Infectious agents and disease. ,vol. 2, pp. 227- ,(1993)
B. B. Finlay, Molecular and Cellular Mechanisms of Salmonella Pathogenesis Current Topics in Microbiology and Immunology. ,vol. 192, pp. 163- 185 ,(1994) , 10.1007/978-3-642-78624-2_8
David Botstein, Ronald W. Davis, John R. Roth, Advanced bacterial genetics Cold Spring Harbor Laboratory. ,(1980)
Stéphane Méresse, Olivia Steele-Mortimer, Edgardo Moreno, Michel Desjardins, Brett Finlay, Jean-Pierre Gorvel, None, Controlling the maturation of pathogen-containing vacuoles: a matter of life and death. Nature Cell Biology. ,vol. 1, ,(1999) , 10.1038/15620