The effect of cell subset isolation method on gene expression in leukocytes.

摘要: Multiple scientific disciplines require the isolation of specific subsets blood cells from patient samples for gene expression analysis by microarray or RNA-sequencing, preserving disease- treatment-related signatures. However, little is known with respect to impact different cell methods on and effects positive selection, negative fluorescence activated sorting (FACS) have not previously been assessed in parallel. To address this knowledge gap, CD4+ T cells, CD8+ B monocytes were isolated five independent donors using immunomagnetic FACS. We hypothesized that selection FACS would yield higher purity but may an since both utilize antibodies bind surface receptors type interest. Moreover, might upregulate stress response genes due passage through sorter. Microarray data generated subjected unsupervised clustering differential analysis. Surprisingly, these analyses revealed signatures more similar between compared selection. are involved generally had highest Thus, recommended method leukocyte studies results purest subset populations does appear induce a response.