The effects of acellular amniotic membrane matrix on osteogenic differentiation and ERK1/2 signaling in human dental apical papilla cells.
作者： Yi-Jane Chen , Min-Chun Chung , Chung-Chen Jane Yao , Chien-Hsun Huang , Hao-Hueng Chang
DOI: 10.1016/J.BIOMATERIALS.2011.09.065
关键词:
摘要: The amniotic membrane (AM) has been widely used in the field of tissue engineering because favorable biological properties for scaffolding material. However, little is known about effects an acellular AM matrix on osteogenic differentiation mesenchymal stem cells. In this study, it was found that both basement side and collagenous stroma were capable providing a preferential environment driving human dental apical papilla cells (APCs) with proven cell characteristics. Acellular potentiated induction effect supplements (OS) such as ascorbic acid, β-glycerophosphate, dexamethasone enhanced APCs, seen by increased core-binding factor alpha 1 (Cbfa-1) phosphorylation, alkaline phosphatase activity, mRNA expression marker genes, mineralized deposition. Even absence soluble OS, also could exert substrate-induced initiating APCs' differentiation. Especially, more effective than side. Moreover, AM-induced significantly inhibited U0126, inhibitor extracellular signaling-regulated kinase 1/2 (ERK1/2) signaling. Taken together, promoting APCs AM-specific, which provides potential applications bone/tooth engineering.
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