Network mapping of the conformational heterogeneity of SOD1 by deploying statistical cluster analysis of FTIR spectra.
作者： Sourav Chowdhury , Sagnik Sen , Amrita Banerjee , Vladimir N. Uversky , Ujjwal Maulik
DOI: 10.1007/S00018-019-03108-2
关键词:
摘要: A crucial contribution to the heterogeneity of conformational landscape a protein comes from way an intermediate relates another state in its journey unfolded folded or misfolded form. Unfortunately, it is extremely hard decode this relatedness quantifiable manner. Here, we developed application statistical cluster analyses explore metalloenzyme, human cytosolic copper–zinc superoxide dismutase (SOD1), using inputs infrared spectroscopy. This study provides picture how information at one particular site (for example, copper-binding pocket) related second zinc-binding pocket), and transferred global protein. The distance outputs were used quantitatively generate network capturing folding sub-stages SOD1.
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