Fluorescence-based assays.

摘要: Publisher Summary Fluorescence techniques occupy a central position in the study of protein–ligand and protein–protein interactions. Knowledge origins various fluorescence parameters described this chapter allows sensitive informative assays to be designed, which can used detailed mechanistic evaluations interactions at equilibrium, as well kinetics enzyme–catalyzed processes. The development accurate multi-well plate readers diversity long wavelength fluorophores have revolutionized use fluorescence-based miniaturized high throughput screening; these methods now underpin majority early stage drug discovery programs.