Neuropeptide precursor gene discovery in the Chagas disease vector Rhodnius prolixus.

摘要: We show a straightforward workflow combining homology search in Rhodnius prolixus genome sequence with cloning by rapid amplification of cDNA ends and mass spectrometry. have identified 32 genes their transcripts that encode number neuropeptide precursors leading to 194 putative peptides. validated spectrometry 82 those predicted neuropeptides the brain R. achieve first comprehensive genomic, transcriptomic neuropeptidomic analysis an insect disease vector. Comparisons available sequences revealed contains most conserved insects, many them displaying specific features at level. Some gene families reported here are for time order Hemiptera, highly biodiverse group insects includes human, animal plant agents.