Temporal pattern of expression and colocalization of microglia/macrophage phenotype markers following brain ischemic injury in mice

摘要: Emerging evidence indicates that, similarly to what happens for peripheral macrophages, microglia can express different phenotypes depending on microenvironmental signals. In spite of the large literature inflammation after ischemia, information M/M phenotype marker expression, their colocalization and temporal evolution in injured brain is lacking. The present study investigates presence microglia/macrophage markers, whether they are concomitantly expressed by same subpopulation, or at distinct phases locations relation ischemic lesion. Volume lesion, neuronal counts TUNEL staining were assessed C57Bl/6 mice 6-12-24-48 h 7d permanent occlusion middle cerebral artery. At time points, distribution lesioned area, association with a definite morphology coexpression markers CD11b, CD45, CD68, Ym1, CD206 immunostaining confocal microscopy. results show that: 1) lesion induces expression selected that develop over time, each specific pattern; 2) has given localization area no apparent changes during exception CD68 confined border zone early times but it greatly increases invades core 7d; 3) while both ramified globular CD11b cells, Ym1 exclusively cells. These data accompanied activation presents distinctive spatial features. states microglia/macrophages reflect complexity these cells ability differentiate towards multitude surrounding micro-environmental signals change time. presented this provide basis understanding complex response developing strategies resulting promotion protective inflammatory phenotype.