Sequence and expression analysis of the cytoplasmic pattern recognition receptor melanoma differentiation-associated gene 5 from the barbel chub Squaliobarbus curriculus

作者: Yaoguo Li , Shengzhen Jin , Xin Zhao , Hong Luo , Rui Li

DOI: 10.1016/J.FSI.2019.08.077

关键词:

摘要: MDA5 is a cytoplasmic viral double-stranded RNA recognition receptor that plays pivotal role in the aquatic animal innate immune system. To decipher of Squaliobarbus curriculus (ScMDA5) response, full-length cDNA ScMDA5 was cloned using RACE technology, mRNA and protein expression levels signalling pathway members response to stimulation were detected effects overexpression on investigated. comprises 3597 bp composed an open reading frame (2958 nucleotides long) translates into putative peptide 985 amino acid residues. possesses two N-terminal caspase-recruiting domains, DEAD-like helicases superfamily, helicase superfamily C-terminal RIG-I_C-RD differences these domains among species mainly observed with respect their length location. closely clustered those Carassius auratus, Ctenopharyngodon idellus Mylopharyngodon piceus. transcripts most abundant spleen lowest liver. Expression healthy tissues significantly correlated ScIRF3, ScIRF7 ScIFN. Besides, ScIRF3 (0.956, P < 0.01). level changes, including downregulation, upregulation initial followed by found molecules after grass carp reovirus infection. Protein highest liver tissues. Overexpression led enhanced CiIRF7 CiMx transcription ovary cells (P < 0.05). The results this study helped clarify reaction against provided fundamental information for fish breeding achieve strong resistance

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