Ca2+ waves are organized among hepatocytes in the intact organ

摘要: Hormone-induced increases in cytosolic Ca2+ (Cai2+) begin as Cai2+ waves cells isolated from most types of tissue (1, 11), but whether such actually occur vivo is unknown. To investigate this, we examined vasopressin-induced signals hepatocytes within the perfused rat liver. Using confocal fluorescence video microscopy, found that began usually originated near central venules, then spread opposite to direction blood flow, portal venules. We used immunochemistry determine liver vasopressin V1a receptor concentrated among pericentral region, providing mechanism by which originate there. Pericentral-to-periportal may direct peristaltic flow bile, since induces contraction apical pole and contractions also a pericentral-to-periportal direction. The organization intact be means an integrative, organ-level response provided hormonal stimuli.