Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle

作者: Aurelijus Zimkus , Audrius Misiūnas , Arūnas Ramanavičius , Larisa Chaustova

DOI: 10.1007/978-3-319-10142-2_23

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摘要: Capability of taking up exogenous DNA yeast Saccharomyces cerevisiae cells was studied by lipophilic cations accumulation and FT-IR spectroscopy. The increase in permeability the treated with Li+ ions observed. changes cell wall structure detected spectra were associated transformability cells. presence a population buds higher to TPP determined. Relationship between transformation efficiency cycle Yeast S-phase showed an enhanced perception DNA. An physiological properties phenomenon natural competence is supposed occur

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