Effects of insulin concentration and self-association on the partitioning of its A-21 cyclic anhydride intermediate to desamido insulin and covalent dimer.
作者： Richard T. Darrington , Bradley D. Anderson
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摘要: Purpose. In the pH range 2–5, human insulin degrades via deamidation at A-21 asn and covalent dimerization. Both products form a common cyclic anhydride intermediate, product of intramolecular nucleophilic attack by carboxyl terminus. This study examines influence [insulin] self-association on partitioning intermediate to products.
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