A synthetic weak neurotoxin binds with low affinity to Torpedo and chicken α7 nicotinic acetylcholine receptors
作者： Siew Lay Poh , Gilles Mourier , Robert Thai , Arunmozhiarasi Armugam , Jordi Molgó
DOI: 10.1046/J.1432-1033.2002.03113.X
关键词:
摘要: Weak neurotoxins from snake venom are small proteins with five disulfide bonds, which have been shown to be poor binders of nicotinic acetylcholine receptors. We report on the cloning and sequencing four cDNAs encoding weak Naja sputatrix glands. The protein encoded by one them, Wntx-5, has synthesized solid-phase synthesis characterized. physicochemical properties synthetic toxin (sWntx-5) agree those anticipated for natural toxin. show that this interacts relatively low affinity (Kd = 180 nm) muscular-type receptor electric organ T. marmorata, an even weaker (90 µm) neuronal α7 chicken. Electrophysiological recordings using isolated mouse hemidiaphragm frog cutaneous pectoris nerve–muscle preparations revealed no blocking activity sWntx-5 at µm concentrations. Our data confirm previous observations cobras
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