Redox Regulation of Ap-1

摘要: The inducible transcription factor, AP-1, is a heterodimeric leucine zipper complex containing the protein products of thefos and jun protooncogenes. DNA binding activity Fos Jun regulated in vitro by posttranslational mechanism involving reduction-oxidation. Redox regulation mediated through conserved cysteine residue located domain both proteins. Oxidation or chemical modification has an inhibitory effect on AP-1 activity. Conversely, reduction this reducing agents ubiquitous nuclear redox factor (Ref-1), purified cloned from human cells, stimulates In addition, recombinant Ref-1 several other classes factors. Immunodepletion studies indicate that major Hela cells. Interestingly, bifunctional protein; it also possesses apurinic/apyrimidinic (A/P) endonuclease repair However, activities are physically biochemically distinguishable. may represent novel component signal transduction processes regulate eukaryotic gene expression response to cellular stress.